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Gram stain quiz

As a member, you'll also get unlimited access to over 79, lessons in math, English, science, history, and more. Plus, get practice tests, quizzes, and personalized coaching to help you succeed. This quiz and worksheet combination will help you test your knowledge of the Gram stain method, the theory behind it, and the actual procedure for performing this type of analysis.

The questions on the quiz will test you on your understanding of gram-positive and gram-negative bacteria, the major components of the Gram stain, and the importance of mordants. To learn more about the Gram stain theory, review the accompanying lesson on these topics.

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gram stain quiz

Sign Up. Explore over 4, video courses. Find a degree that fits your goals. Try it risk-free for 30 days. Instructions: Choose an answer and hit 'next'.

You will receive your score and answers at the end. Purple Red Green. Yellow Colorless. Cancel anytime. Gram-negative bacteria appear. A mordant is. A substance that enhances staining ability. Create your account to access this entire worksheet. Create an account to get started Create Account. This lesson, called The Gram Stain: Theory and Procedure, covers the following objectives: Define the Gram stain method Discuss the procedure for performing the Gram stain method of bacterial investigation Define the nine-step process for performing the Gram stain.

Practice Exams. Final Exam. Microbiology Intro to Microbiology. Microbiology Laboratory Techniques. You are viewing lesson Lesson 3 in chapter 4 of the course:. Biology Review. Microbiology Basics. Bacterial Biology. Microorganisms and the The Disease Process. Protozoan Diseases. Introduction to Viruses. DNA Viruses. Ch RNA Viruses. Fungal Infections.Gram-positive bacteria are bacteria with thick cell walls. In a Gram stain testthese organisms yield a positive result.

Though both groups of bacteria can cause disease, they require different treatments. If you have a bacterial infection, the Gram stain will determine what kind of medication you need.

Read on to learn about gram-positive bacteria and their associated diseases, along with typical treatments. The hallmark trait of gram-positive bacteria is their structure.

Generally, they have the following characteristics:. Gram-positive and gram-negative bacteria have different structures. Typically, gram-negative organisms have the following the traits:. The major difference is the outer lipid membrane. Because of this difference, gram-negative bacteria are harder to kill. This means gram-positive and gram-negative bacteria require different treatments.

Though gram-negative bacteria are harder to destroy, gram-positive bacteria can still cause problems. Many species result in disease and require specific antibiotics.

Gram stain testing is a method for classifying bacteria based on their cell wall. It allows scientists to determine whether an organism is gram-positive or gram-negative. The test, which uses a microscope, was created by Hans Christian Gram in During the procedure, crystal violet dye is applied to a sample of bacteria.

gram stain quiz

This chemical dye can stain thick peptidoglycan layers. Under a microscope, gram-positive bacteria appear purple-blue because their thick peptidoglycan membrane can hold the dye. The bacteria is called gram-positive due to the positive result. Gram-negative bacteria stain pink-red.

The test result is negative.Maintained by BE4U. Multiple Choice Questions on Gram Staining. Gram staining was developed by. Gram staining is an example of.

Gram Stain Procedure in Microbiology

Gram staining was developed in. The most common stains used in Gram staining is. Which of the following statements are true regarding Gram positive bacteria. Which of the following statements is incorrect regarding Gram negative bacteria. In Gram stainingif some bacteria retain the crystal violet stain after alcohol treatment.

Then the bacteria is. Counter stain used in Gram staining is. In Gram stainingthe alcohol acts on. Lipopolysaccharide is found in cell wall of. In Gram staining iodine is used as a. After ethanol treatment Gram negative bacteria can be visualised. W hich of the following is a common Gram positive bacteria. During ethanol treatment in gram staining.

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gram stain quiz

Search here. Autosomal Dominant vs X linked Dominant Pedigree analysis. Follow by Email. Thanks for visiting this site. Enjoy Biology. Bacterial cell wall.To detect the presence and identify the general type of bacteria or sometimes fungi microbes in a sample taken from the site of a suspected infection; to generally classify bacteria grown in culture so that further identification tests can be performed and appropriate treatment given.

When a healthcare practitioner suspects that you have a bacterial or sometimes fungal infection; often whenever a culture is requested. Pusbody fluid, sputumor swab of cells taken from the site of an infection; a sample of bacteria or fungi grown and isolated in culture.

You may be able to find your test results on your laboratory's website or patient portal. However, you are currently at Lab Tests Online. You may have been directed here by your lab's website in order to provide you with background information about the test s you had performed.

Lab Tests Online is an award-winning patient education website offering information on laboratory tests. The reference ranges for your tests can be found on your laboratory report.

The Gram Stain: Background and Example Organisms

They are typically found to the right of your results. If you do not have your lab report, consult your healthcare provider or the laboratory that performed the test s to obtain the reference range. Laboratory test results are not meaningful by themselves. Their meaning comes from comparison to reference ranges. Reference ranges are the values expected for a healthy person.

They are sometimes called "normal" values. By comparing your test results with reference values, you and your healthcare provider can see if any of your test results fall outside the range of expected values. Values that are outside expected ranges can provide clues to help identify possible conditions or diseases.

While accuracy of laboratory testing has significantly evolved over the past few decades, some lab-to-lab variability can occur due to differences in testing equipment, chemical reagents, and techniques. This is a reason why so few reference ranges are provided on this site. It is important to know that you must use the range supplied by the laboratory that performed your test to evaluate whether your results are "within normal limits.

It gives relatively quick results as to whether bacteria or fungi are present and, if so, the general type s. The Gram stain involves applying a sample from the infected area onto a glass slide and allowing it to dry. The slide is then treated with a special stain and examined under a microscope by a trained laboratorian. Any bacteria that may be present are categorized by color and shape during the microscopic evaluation:. Additional information may be obtained by observing the groupings of the bacteria on the slide, such as cocci that are present singly, in pairs, in groups of four, in clusters or in chains, or bacilli that are thick, thin, short, long, or have enlarged spores on one end.

Any bacteria that are present within white blood cells intracellular are also noted. The Gram stain color and the bacterial shape give clues as to what bacteria might be causing the infection. An example of gram-negative bacteria is Escherichia colithe cause of many urinary tract infections.

Fungi in the form of yeasts or molds can also be initially recognized with the Gram stain, but viruses cannot be seen with a Gram stain. Though Gram stains are useful as initial tests for detecting and identifying general types of bacteria or fungi, results are usually considered preliminary.

Sometimes, susceptibility testing is necessary to determine which antibiotic will be most effective in treating the infection. Several different types of samples may be collected for Gram stains. Some samples are collected using sterile swabs to obtain cells or exudate at the site of a suspected infection. Other samples, such as urine or sputummay be collected in a sterile container. Some body fluids may be collected by needle and syringe. A swab may be used to collect a sample of bacteria grown and isolated in a culture.

A Gram stain is used, along with a culture of the material from an infected site, to identify the cause of a bacterial infection. The Gram stain provides preliminary results on whether bacteria are present and the general type, such as the shape and whether they are Gram-positive or Gram-negative.

Often, detecting the presence of bacteria and determining whether an infection is caused by a microbe that is Gram-positive or Gram-negative will be sufficient to allow a healthcare practitioner to prescribe treatment with an appropriate antibiotic while waiting for more specific tests, such as a culture, to be completed. Absence or presence of white blood cells in the Gram stain can help establish that an adequate sample was obtained as white blood cells are frequently present with an infection.

Gram Staining: Principle, Procedure and Results

A Gram stain may also be performed as part of the evaluation of a culture.Last reviewed by Editorial Team on February 9th, Gram staining is a microbiologic procedure used to differentiate Gram-negative from Gram-positive bacteria. It was developed by Hans Christian Gram; a Danish physician, in The cells are colored red or violet so as to distinguish its group. A microorganism is gram-positive if the stain remains violet secondary to the presence of peptidoglycan in the cell wall.

On the other hand, a microorganism is gram-negative if the stain is red secondary to the thin peptidoglycan wall. Picture 1 : The image shows the steps needed to complete the gram staining procedure. Picture Source: vignette.

Picture 2: A gram stain procedure showed gram positive bacteria on the left and gram negative bacteria on the right. Photo 3: Gram positive bacteria remain purple in color while gram negative bacteria turn pink. Picture 4 : Gram staining and the steps needed to complete the procedure. Gram staining and the steps Picture Source: www. The composition of the cellular wall of gram-positive and gram-negative bacteria tells the gram staining differences.

The following principles apply:. The gram-staining procedure is one of the most important procedures in microbiology. Its primary purpose is to differentiate gram-positive from gram-negative bacteria. Identifying and classifying bacteria are important as they help in the presumptive diagnosis of various types of diseases. It also helps in understanding the chemical makeup of the cell wall of bacteria which is helpful in determining the course of treatment for a specific type of disease.

The result of the test can help the doctor in identifying the type of bacteria that cause the infection and determining the appropriate treatment plan. Your Name. Go search.If your doctor suspects that you have a bacterial infection; often whenever a culture is requested. Pus, blood or other body fluid, sputum, or a swab of cells taken from the site of infection; a sample of a microorganism grown and isolated in culture.

It gives relatively quick results as to the general type of bacteria that may be present. The slide is then treated with a series of special stains and examined under a microscope by trained laboratory staff. It is based on the principle that most bacteria are visualised under the microscope more easily after staining, which gives them a colour, depending on the type of cell wall.

Any bacteria that may be present are categorised by colour and shape during the microscopic evaluation:. Additional information may be obtained by observing the groupings of the bacteria on the slide, such as cocci that are present singly, in pairs, in groups of four, in clusters or in chains, or bacilli that are thick, thin, short, long or have enlarged spores on one end.

Any bacteria that are present within the patient's white blood cell intracellular are also noted. The Gram stain colour and the bacterial shape give clues as to what microorganism might be causing the infection.

Examples of Gram positive cocci include Staphylococcus aureusthe bacterium commonly known as "Golden Staph". Fungi in the form of yeasts or moulds may also be initially recognised with a Gram stain, but viruses cannot be seen with a Gram stain.

Since its discovery in by Danish scientist Hans Christian Gram, the Gram stain remains the most simple, fast and cost-effective test in microbiology, providing an incredible amount of information to health practitioners.

Particularly in patients who are suspected of septicaemia, pneumonia and meningitis. Several different types of samples may be collected for Gram stains. Other samples, such as urine or sputummay be collected in a sterile container. Some body fluids may be collected by needle and syringe.

A swab may be used to collect a sample of bacteria grown and isolated in a culture. A Gram stain and culture of the material from an infected site are the most commonly performed microbiology tests used to identify the cause of an infection. Often, detecting the presence of microorganisms and determining whether an infection is caused by an organism that is Gram positive or Gram negative will be sufficient to allow a doctor to prescribe treatment with an appropriate empiric antibiotic while waiting for more specific tests, such as culture and antibiotic sensitivities, to be completed.

Absence or presence of white blood cells in the Gram stain can help establish that an adequate sample was obtained as white blood cells are frequently present with an infection. With the exception of vaginal specimens, absence or presence of skin cells epithelial cells may represent a contaminated sample or a poor-quality specimen.

Careful interpretation of the cells, and or bacteria shown in a Gram stain allow the health practitioners to differentiate between normal flora and true infection. A Gram stain is often routinely performed as part of the evaluation of a culture. It is performed on the same sample as the culture, and the test results are reported out promptly to help guide treatment.

A negative Gram stain is often reported as "no bacteria seen". This may mean that there is no bacterial infection present or that there were not enough microorganisms present in the sample to be seen with the stain under a microscope. Positive Gram stain results usually include a description of what was seen on the slide.

This typically includes whether the bacteria are Gram-positive purple or Gram-negative pink as well as their shape — round cocci or rods bacilli. Sometimes size, relative quantity and grouping of the bacteria, if relevant, are also reported. If there are bacteria present within other cells intracellularthis will also be noted as well as; for example, the presence of red blood cells or white blood cells.

gram stain quiz

They may initially be present in low numbers with an infection, and a sample may require extra processing in order to concentrate the bacteria so that they can be detected by a Gram stain. Gram stains on these types of samples require careful examination by a trained laboratory scientist to determine specific features that indicate infection.

If left untreated, bacterial infections can spread and may eventually cause tissue and organ damage. Prompt treatment can limit their spread and severity. Yeast may appear as single cells that may have buds, while moulds may appear as a wide variety of plant-like branches called hyphae.

Parasites and viruses are not distinguished by Gram staining. Some bacteria are not able to be identified properly using the Gram stain.QUES 1. The Gram staining technique was developed by H. Gram N. Gram H. Gram A. Gram Correct! My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life.

Your email address will not be published. Save my name, email, and website in this browser for the next time I comment. Skip to content. The Gram staining technique was developed by.

QUES 2. Mordant used in grams staining is. Crystal Violet. All of the Above. QUES 3. Gram staining is an example of. Simple Staining. Capsule staining. Differential Staining.

PlantEd Digital Learning Library - Gram Stain Procedure

None of the Above. QUES 4. Iodine solution, Crystal Violet, Safranine, Alcohol. Alcohol, Crystal Violet, Iodine solution, Safranine. Crystal violet, Iodine solution, Alcohol, Safranine.

Not Mentioned here. QUES 5. The percentage of alcohol used in Gram's Staining is. QUES 6. The action of alcohol during Grams Staining is. It adds color.


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Vilkree Posted on 10:12 pm - Oct 2, 2012

Es ist die Bedingtheit, weder es ist mehr, noch weniger